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proteon microplate sealing film  (Bio-Rad)


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    Structured Review

    Bio-Rad proteon microplate sealing film
    ( A ) Surface plasmon resonance data for compound H1 binding to PcrV at 400, 200 (duplicate), 100, 50, 25, 12.5, 6.25, and 0 µM. ( B ) Equilibrium analysis of compound H1 from binding levels at 45-55 s in A : K D was determined to be 98.3 µM. R max was 71.3 RU, and Chi 2 was 10.0 RU. ( C ) Infection assay with dose-response analysis of compound H1 that protects macrophages from T3SS-mediated P. aeruginosa toxicity (wt): uninfected cells and infection with a pcrV mutant were used as controls, and standard deviation was calculated by Gaussian approximation. ( D ) 1 H NMR relaxation-edited binding experiment showing the aromatic region for compound H1 at 100 µM with (bottom trace) and without 10 µM PcrV (upper trace): equilibrium analysis was performed in the <t>ProteOn</t> Manager software TM (Bio-Rad Laboratories Inc., Hercules, CA, USA). ( E ) Bacterial growth assay where P. aeruginosa was grown in the presence of compound H1 at 200, 100, 50, and 25 µM and OD600 was measured regularly for 8 h: each value represents the mean value of a triplicate; to the control wells, 1% DMSO was added (DMSO). The panels show one representative experiment.
    Proteon Microplate Sealing Film, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteon microplate sealing film/product/Bio-Rad
    Average 92 stars, based on 2 article reviews
    proteon microplate sealing film - by Bioz Stars, 2026-02
    92/100 stars

    Images

    1) Product Images from "Identification of Small Molecules Blocking the Pseudomonas aeruginosa Type III Secretion System Protein PcrV"

    Article Title: Identification of Small Molecules Blocking the Pseudomonas aeruginosa Type III Secretion System Protein PcrV

    Journal: Biomolecules

    doi: 10.3390/biom11010055

    ( A ) Surface plasmon resonance data for compound H1 binding to PcrV at 400, 200 (duplicate), 100, 50, 25, 12.5, 6.25, and 0 µM. ( B ) Equilibrium analysis of compound H1 from binding levels at 45-55 s in A : K D was determined to be 98.3 µM. R max was 71.3 RU, and Chi 2 was 10.0 RU. ( C ) Infection assay with dose-response analysis of compound H1 that protects macrophages from T3SS-mediated P. aeruginosa toxicity (wt): uninfected cells and infection with a pcrV mutant were used as controls, and standard deviation was calculated by Gaussian approximation. ( D ) 1 H NMR relaxation-edited binding experiment showing the aromatic region for compound H1 at 100 µM with (bottom trace) and without 10 µM PcrV (upper trace): equilibrium analysis was performed in the ProteOn Manager software TM (Bio-Rad Laboratories Inc., Hercules, CA, USA). ( E ) Bacterial growth assay where P. aeruginosa was grown in the presence of compound H1 at 200, 100, 50, and 25 µM and OD600 was measured regularly for 8 h: each value represents the mean value of a triplicate; to the control wells, 1% DMSO was added (DMSO). The panels show one representative experiment.
    Figure Legend Snippet: ( A ) Surface plasmon resonance data for compound H1 binding to PcrV at 400, 200 (duplicate), 100, 50, 25, 12.5, 6.25, and 0 µM. ( B ) Equilibrium analysis of compound H1 from binding levels at 45-55 s in A : K D was determined to be 98.3 µM. R max was 71.3 RU, and Chi 2 was 10.0 RU. ( C ) Infection assay with dose-response analysis of compound H1 that protects macrophages from T3SS-mediated P. aeruginosa toxicity (wt): uninfected cells and infection with a pcrV mutant were used as controls, and standard deviation was calculated by Gaussian approximation. ( D ) 1 H NMR relaxation-edited binding experiment showing the aromatic region for compound H1 at 100 µM with (bottom trace) and without 10 µM PcrV (upper trace): equilibrium analysis was performed in the ProteOn Manager software TM (Bio-Rad Laboratories Inc., Hercules, CA, USA). ( E ) Bacterial growth assay where P. aeruginosa was grown in the presence of compound H1 at 200, 100, 50, and 25 µM and OD600 was measured regularly for 8 h: each value represents the mean value of a triplicate; to the control wells, 1% DMSO was added (DMSO). The panels show one representative experiment.

    Techniques Used: SPR Assay, Binding Assay, Infection, Mutagenesis, Standard Deviation, Software, Growth Assay, Control



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    ( A ) Surface plasmon resonance data for compound H1 binding to PcrV at 400, 200 (duplicate), 100, 50, 25, 12.5, 6.25, and 0 µM. ( B ) Equilibrium analysis of compound H1 from binding levels at 45-55 s in A : K D was determined to be 98.3 µM. R max was 71.3 RU, and Chi 2 was 10.0 RU. ( C ) Infection assay with dose-response analysis of compound H1 that protects macrophages from T3SS-mediated P. aeruginosa toxicity (wt): uninfected cells and infection with a pcrV mutant were used as controls, and standard deviation was calculated by Gaussian approximation. ( D ) 1 H NMR relaxation-edited binding experiment showing the aromatic region for compound H1 at 100 µM with (bottom trace) and without 10 µM PcrV (upper trace): equilibrium analysis was performed in the <t>ProteOn</t> Manager software TM (Bio-Rad Laboratories Inc., Hercules, CA, USA). ( E ) Bacterial growth assay where P. aeruginosa was grown in the presence of compound H1 at 200, 100, 50, and 25 µM and OD600 was measured regularly for 8 h: each value represents the mean value of a triplicate; to the control wells, 1% DMSO was added (DMSO). The panels show one representative experiment.
    Proteon Microplate Sealing Film, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) Surface plasmon resonance data for compound H1 binding to PcrV at 400, 200 (duplicate), 100, 50, 25, 12.5, 6.25, and 0 µM. ( B ) Equilibrium analysis of compound H1 from binding levels at 45-55 s in A : K D was determined to be 98.3 µM. R max was 71.3 RU, and Chi 2 was 10.0 RU. ( C ) Infection assay with dose-response analysis of compound H1 that protects macrophages from T3SS-mediated P. aeruginosa toxicity (wt): uninfected cells and infection with a pcrV mutant were used as controls, and standard deviation was calculated by Gaussian approximation. ( D ) 1 H NMR relaxation-edited binding experiment showing the aromatic region for compound H1 at 100 µM with (bottom trace) and without 10 µM PcrV (upper trace): equilibrium analysis was performed in the <t>ProteOn</t> Manager software TM (Bio-Rad Laboratories Inc., Hercules, CA, USA). ( E ) Bacterial growth assay where P. aeruginosa was grown in the presence of compound H1 at 200, 100, 50, and 25 µM and OD600 was measured regularly for 8 h: each value represents the mean value of a triplicate; to the control wells, 1% DMSO was added (DMSO). The panels show one representative experiment.
    Idhii, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Surface plasmon resonance data for compound H1 binding to PcrV at 400, 200 (duplicate), 100, 50, 25, 12.5, 6.25, and 0 µM. ( B ) Equilibrium analysis of compound H1 from binding levels at 45-55 s in A : K D was determined to be 98.3 µM. R max was 71.3 RU, and Chi 2 was 10.0 RU. ( C ) Infection assay with dose-response analysis of compound H1 that protects macrophages from T3SS-mediated P. aeruginosa toxicity (wt): uninfected cells and infection with a pcrV mutant were used as controls, and standard deviation was calculated by Gaussian approximation. ( D ) 1 H NMR relaxation-edited binding experiment showing the aromatic region for compound H1 at 100 µM with (bottom trace) and without 10 µM PcrV (upper trace): equilibrium analysis was performed in the ProteOn Manager software TM (Bio-Rad Laboratories Inc., Hercules, CA, USA). ( E ) Bacterial growth assay where P. aeruginosa was grown in the presence of compound H1 at 200, 100, 50, and 25 µM and OD600 was measured regularly for 8 h: each value represents the mean value of a triplicate; to the control wells, 1% DMSO was added (DMSO). The panels show one representative experiment.

    Journal: Biomolecules

    Article Title: Identification of Small Molecules Blocking the Pseudomonas aeruginosa Type III Secretion System Protein PcrV

    doi: 10.3390/biom11010055

    Figure Lengend Snippet: ( A ) Surface plasmon resonance data for compound H1 binding to PcrV at 400, 200 (duplicate), 100, 50, 25, 12.5, 6.25, and 0 µM. ( B ) Equilibrium analysis of compound H1 from binding levels at 45-55 s in A : K D was determined to be 98.3 µM. R max was 71.3 RU, and Chi 2 was 10.0 RU. ( C ) Infection assay with dose-response analysis of compound H1 that protects macrophages from T3SS-mediated P. aeruginosa toxicity (wt): uninfected cells and infection with a pcrV mutant were used as controls, and standard deviation was calculated by Gaussian approximation. ( D ) 1 H NMR relaxation-edited binding experiment showing the aromatic region for compound H1 at 100 µM with (bottom trace) and without 10 µM PcrV (upper trace): equilibrium analysis was performed in the ProteOn Manager software TM (Bio-Rad Laboratories Inc., Hercules, CA, USA). ( E ) Bacterial growth assay where P. aeruginosa was grown in the presence of compound H1 at 200, 100, 50, and 25 µM and OD600 was measured regularly for 8 h: each value represents the mean value of a triplicate; to the control wells, 1% DMSO was added (DMSO). The panels show one representative experiment.

    Article Snippet: The plates were sealed (ProteOn Microplate sealing film, Bio-Rad Laboratories Inc., Hercules, CA, USA) when the plate was prepared.

    Techniques: SPR Assay, Binding Assay, Infection, Mutagenesis, Standard Deviation, Software, Growth Assay, Control